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detection antibodies against total atm  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology detection antibodies against total atm
    <t>ATM</t> inhibition by M3541 circumvents the M6620-induced G 1 checkpoint, resulting in aberrant cell-cycle progression and cell death. A, Quantification of ATM autophosphorylation (p-ATM Ser1981 <t>/total</t> <t>ATM)</t> in A549 cells by MSD assay after 24 hours exposure to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L), or their combination. Error bars, SEM; ****, P < 0.0001 and ***, P < 0.001 by unpaired t test. B, Western blot analysis of components of the ATM/p53 signaling pathway in A549 cells after 24-hour exposure to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L), or their combination. C, BrdU/7-AAD cell-cycle analysis in A549, A375, and H460 cells after 24 hours after treatment with DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L) or their combination. D, Confluence (top row) and relative cell death (bottom row) from IncuCyte live imaging in A549 parental, p53-null, and ATM-null cells exposed to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L) or their combination. Relative cell death was calculated from the number of cells positively stained with CytoTox dye normalized to confluence. Error bars, SEM. E, Representative images from IncuCyte live imaging (10× objective) of A549 parental, p53-null, and ATM-null cells after 6-day exposure to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L), or their combination. Scale bars = 100 μmol/L. F, Bliss synergy plots generated from CellTiter-Glo assay viability results. A549 paired isogenic cell lines (parental and ATM-null) were treated for 5 days with titrations of M3541 and M6620. Bliss synergy was determined using Combenefit software.
    Detection Antibodies Against Total Atm, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 359 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/detection+antibodies+against+total+atm/Atm+Antibody/pmc10320480-91-15-20
    Average 94 stars, based on 359 article reviews
    detection antibodies against total atm - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Selective Inhibition of ATM-dependent Double-strand Break Repair and Checkpoint Control Synergistically Enhances the Efficacy of ATR Inhibitors"

    Article Title: Selective Inhibition of ATM-dependent Double-strand Break Repair and Checkpoint Control Synergistically Enhances the Efficacy of ATR Inhibitors

    Journal: Molecular Cancer Therapeutics

    doi: 10.1158/1535-7163.MCT-22-0685

    ATM inhibition by M3541 circumvents the M6620-induced G 1 checkpoint, resulting in aberrant cell-cycle progression and cell death. A, Quantification of ATM autophosphorylation (p-ATM Ser1981 /total ATM) in A549 cells by MSD assay after 24 hours exposure to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L), or their combination. Error bars, SEM; ****, P < 0.0001 and ***, P < 0.001 by unpaired t test. B, Western blot analysis of components of the ATM/p53 signaling pathway in A549 cells after 24-hour exposure to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L), or their combination. C, BrdU/7-AAD cell-cycle analysis in A549, A375, and H460 cells after 24 hours after treatment with DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L) or their combination. D, Confluence (top row) and relative cell death (bottom row) from IncuCyte live imaging in A549 parental, p53-null, and ATM-null cells exposed to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L) or their combination. Relative cell death was calculated from the number of cells positively stained with CytoTox dye normalized to confluence. Error bars, SEM. E, Representative images from IncuCyte live imaging (10× objective) of A549 parental, p53-null, and ATM-null cells after 6-day exposure to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L), or their combination. Scale bars = 100 μmol/L. F, Bliss synergy plots generated from CellTiter-Glo assay viability results. A549 paired isogenic cell lines (parental and ATM-null) were treated for 5 days with titrations of M3541 and M6620. Bliss synergy was determined using Combenefit software.
    Figure Legend Snippet: ATM inhibition by M3541 circumvents the M6620-induced G 1 checkpoint, resulting in aberrant cell-cycle progression and cell death. A, Quantification of ATM autophosphorylation (p-ATM Ser1981 /total ATM) in A549 cells by MSD assay after 24 hours exposure to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L), or their combination. Error bars, SEM; ****, P < 0.0001 and ***, P < 0.001 by unpaired t test. B, Western blot analysis of components of the ATM/p53 signaling pathway in A549 cells after 24-hour exposure to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L), or their combination. C, BrdU/7-AAD cell-cycle analysis in A549, A375, and H460 cells after 24 hours after treatment with DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L) or their combination. D, Confluence (top row) and relative cell death (bottom row) from IncuCyte live imaging in A549 parental, p53-null, and ATM-null cells exposed to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L) or their combination. Relative cell death was calculated from the number of cells positively stained with CytoTox dye normalized to confluence. Error bars, SEM. E, Representative images from IncuCyte live imaging (10× objective) of A549 parental, p53-null, and ATM-null cells after 6-day exposure to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L), or their combination. Scale bars = 100 μmol/L. F, Bliss synergy plots generated from CellTiter-Glo assay viability results. A549 paired isogenic cell lines (parental and ATM-null) were treated for 5 days with titrations of M3541 and M6620. Bliss synergy was determined using Combenefit software.

    Techniques Used: Inhibition, Western Blot, Cell Cycle Assay, Imaging, Staining, Generated, Glo Assay, Software

    Related Articles

    Incubation:

    Article Title: Selective Inhibition of ATM-dependent Double-strand Break Repair and Checkpoint Control Synergistically Enhances the Efficacy of ATR Inhibitors
    Article Snippet: .. Plates were incubated with equal microgram amounts of lysates for 2 hours, incubated with primary detection antibodies against total ATM (Santa Cruz Biotechnology #sc135663 or Abcam #ab199726) for 1 hour, and then incubated with secondary detection antibody (MSD #R32AC-5 for anti-mouse SULFO-Tag and MSD #R32AB-5 for anti-rabbit SULFO-Tag). ..

    Article Title: Selective Inhibition of ATM-dependent Double-strand Break Repair and Checkpoint Control Synergistically Enhances the Efficacy of ATR Inhibitors
    Article Snippet: .. Plates were incubated with equal microgram amounts of lysates for 2 hours, incubated with primary detection antibodies against total ATM (Santa Cruz Biotechnology #sc135663 or Abcam #ab199726) for 1 hour, and then incubated with secondary detection antibody (MSD #R32AC-5 for anti-mouse SULFO-Tag and MSD #R32AB-5 for anti-rabbit SULFO-Tag). ..



    Similar Products

    detection antibodies against total atm  (Santa Cruz Biotechnology)
    94
    Santa Cruz Biotechnology detection antibodies against total atm
    <t>ATM</t> inhibition by M3541 circumvents the M6620-induced G 1 checkpoint, resulting in aberrant cell-cycle progression and cell death. A, Quantification of ATM autophosphorylation (p-ATM Ser1981 <t>/total</t> <t>ATM)</t> in A549 cells by MSD assay after 24 hours exposure to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L), or their combination. Error bars, SEM; ****, P < 0.0001 and ***, P < 0.001 by unpaired t test. B, Western blot analysis of components of the ATM/p53 signaling pathway in A549 cells after 24-hour exposure to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L), or their combination. C, BrdU/7-AAD cell-cycle analysis in A549, A375, and H460 cells after 24 hours after treatment with DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L) or their combination. D, Confluence (top row) and relative cell death (bottom row) from IncuCyte live imaging in A549 parental, p53-null, and ATM-null cells exposed to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L) or their combination. Relative cell death was calculated from the number of cells positively stained with CytoTox dye normalized to confluence. Error bars, SEM. E, Representative images from IncuCyte live imaging (10× objective) of A549 parental, p53-null, and ATM-null cells after 6-day exposure to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L), or their combination. Scale bars = 100 μmol/L. F, Bliss synergy plots generated from CellTiter-Glo assay viability results. A549 paired isogenic cell lines (parental and ATM-null) were treated for 5 days with titrations of M3541 and M6620. Bliss synergy was determined using Combenefit software.
    Detection Antibodies Against Total Atm, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/detection+antibodies+against+total+atm/Atm+Antibody/pmc10320480-91-15-20
    Average 94 stars, based on 1 article reviews
    detection antibodies against total atm - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    ATM inhibition by M3541 circumvents the M6620-induced G 1 checkpoint, resulting in aberrant cell-cycle progression and cell death. A, Quantification of ATM autophosphorylation (p-ATM Ser1981 /total ATM) in A549 cells by MSD assay after 24 hours exposure to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L), or their combination. Error bars, SEM; ****, P < 0.0001 and ***, P < 0.001 by unpaired t test. B, Western blot analysis of components of the ATM/p53 signaling pathway in A549 cells after 24-hour exposure to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L), or their combination. C, BrdU/7-AAD cell-cycle analysis in A549, A375, and H460 cells after 24 hours after treatment with DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L) or their combination. D, Confluence (top row) and relative cell death (bottom row) from IncuCyte live imaging in A549 parental, p53-null, and ATM-null cells exposed to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L) or their combination. Relative cell death was calculated from the number of cells positively stained with CytoTox dye normalized to confluence. Error bars, SEM. E, Representative images from IncuCyte live imaging (10× objective) of A549 parental, p53-null, and ATM-null cells after 6-day exposure to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L), or their combination. Scale bars = 100 μmol/L. F, Bliss synergy plots generated from CellTiter-Glo assay viability results. A549 paired isogenic cell lines (parental and ATM-null) were treated for 5 days with titrations of M3541 and M6620. Bliss synergy was determined using Combenefit software.

    Journal: Molecular Cancer Therapeutics

    Article Title: Selective Inhibition of ATM-dependent Double-strand Break Repair and Checkpoint Control Synergistically Enhances the Efficacy of ATR Inhibitors

    doi: 10.1158/1535-7163.MCT-22-0685

    Figure Lengend Snippet: ATM inhibition by M3541 circumvents the M6620-induced G 1 checkpoint, resulting in aberrant cell-cycle progression and cell death. A, Quantification of ATM autophosphorylation (p-ATM Ser1981 /total ATM) in A549 cells by MSD assay after 24 hours exposure to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L), or their combination. Error bars, SEM; ****, P < 0.0001 and ***, P < 0.001 by unpaired t test. B, Western blot analysis of components of the ATM/p53 signaling pathway in A549 cells after 24-hour exposure to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L), or their combination. C, BrdU/7-AAD cell-cycle analysis in A549, A375, and H460 cells after 24 hours after treatment with DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L) or their combination. D, Confluence (top row) and relative cell death (bottom row) from IncuCyte live imaging in A549 parental, p53-null, and ATM-null cells exposed to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L) or their combination. Relative cell death was calculated from the number of cells positively stained with CytoTox dye normalized to confluence. Error bars, SEM. E, Representative images from IncuCyte live imaging (10× objective) of A549 parental, p53-null, and ATM-null cells after 6-day exposure to DMSO, M3541 (1 μmol/L), M6620 (200 nmol/L), or their combination. Scale bars = 100 μmol/L. F, Bliss synergy plots generated from CellTiter-Glo assay viability results. A549 paired isogenic cell lines (parental and ATM-null) were treated for 5 days with titrations of M3541 and M6620. Bliss synergy was determined using Combenefit software.

    Article Snippet: Plates were incubated with equal microgram amounts of lysates for 2 hours, incubated with primary detection antibodies against total ATM (Santa Cruz Biotechnology #sc135663 or Abcam #ab199726) for 1 hour, and then incubated with secondary detection antibody (MSD #R32AC-5 for anti-mouse SULFO-Tag and MSD #R32AB-5 for anti-rabbit SULFO-Tag).

    Techniques: Inhibition, Western Blot, Cell Cycle Assay, Imaging, Staining, Generated, Glo Assay, Software